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Mediation analysis supports a causal relationship between maternal hyperglycemia and placental DNA methylation variations at the leptin gene locus and cord blood leptin levels

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Reverse transcription (50 C for 30 min) was followed by a pre-amplification step (24 cycles: 95 C for 15 s, 60 C for 4 min) with a mixture of the probes below (each diluted to 0.2 standard working concentration) in a total volume of 9 l

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